Determination of Diphacinone in Grain Samples by Using Adsorptive Stripping Voltammetry

Abstract

A sensitive method for the determination of the rodenticide diphacinone by differential adsorptive voltammetry (Dp-AdSV) on a hanging mercury drop electrode (HMDE) (pH 6.0) was described. The cyclic voltammograms demonstrate the adsorption of diphacinone at the mercury electrode. The optimal operational conditions of the proposed procedure were accumulated potential E_acc = 0.0 V, accumulation time (t_acc) = 60 s, scan rate = 40 mV s^-1, pulse amplitude = 28 mV using a universal buffer pH = 6.0 as a supporting electrolyte. The linear concentration range was found to be 2 x 10^-10 to 3 x 10^-7 mol/L with the lower detection limit of 1 x 10^-10M. The procedure was successfully applied for determination of rodenticides in formulations, food grain samples such as chick pea and maize.