Insoluble Biopolymer Based Metal Oxide Nanocomposites as Matrix for Enzyme Immobilization

Authors

  • Anjali Krishna G.
  • Geetha G.
  • Savitha D. P.
  • Mohanan P. V.

Keywords:

α-amylase, DNS Method, Lowry’s Method, Thermal Stability, Reusability

Abstract

Microbial α-amylase obtained as a result of screening of Bacillus subtilis strains can improve the breakdown of heavier biopolymers into simple sugar units. Insoluble chitosan-MnO2 (CH-MnO2, S1) and chitosan-NiO (CH-NiO, S2) nanocomposites were synthesised as a matrix for the adsorption of α-amylase and facilitate industrial applications. The physico-chemical characterizations of the synthesised nanocomposites were successfully determined. The optimization of immobilization criteria’s such as pH, temperature, contact time and concentration were noticed using Lowry’s method. The comparison of efficiency of free enzyme with immobilized enzyme were carried out by finding out the activity using DNS method followed by the determination of properties of free enzyme and immobilized enzyme. The rate of activity of free and immobilized enzymes were compared on the basis on activity temperature relationship. The kinetic studies revealed that supports S1 and S2 showed higher Km and lower Vm values in comparison with free enzyme. The obtained values for Km and Vm for S1 and S2 are 1.6303 mg/mL, 54 μmolmg-1min-1 and 1.2947 mg/mL, 44.8 μmolmg-1min-1 respectively. Thermal stability studies showed that S1 and S2 can withstand maximum relative activity upto 65 C respectively. The reusability of the immobilized enzymes S1 and S2 showed maximum retainity in relative activity after 8 cycles. Storage stability of immobilized enzymes S1 and S2 were checked weekly and observed good stability upto 56 % for S1 and 66 % for S2 respectively after 6 weeks. The immobilization efficiency calculated for S1 and S2 were 56 % and 82.5 % respectively. The obtained results arrived at a conclusion of having better immobilization efficiency of support S2 over S1.

Downloads

Published

2021-07-16

How to Cite

Anjali Krishna G., Geetha G., Savitha D. P., & Mohanan P. V. (2021). Insoluble Biopolymer Based Metal Oxide Nanocomposites as Matrix for Enzyme Immobilization. International Journal of Research in Chemistry and Environment (IJRCE), 11(2), 1–11. Retrieved from https://ijrce.org/index.php/ijrce/article/view/194

Issue

Section

Articles