One Step Biosynthesis of Gold Nanoparticles Using the Leaf Extract of Gymnema sylvestre and Study of Its In-vitro Anticancer Activity on MCF-7 Cell Lines

Abstract

The leaf extract of Gymnema sylvestre has been utilized for the synthesis of gold nanoparticles (AuNPs) at room temperature under a very mild condition without any additional stabilizing or capping agents.  Mostly spherical shaped AuNPs of   9-11 nm average diameters were obtained.   The stabilized gold nanoparticles were characterized by Surface Plasmon Resonance Spectroscopy, DLS, HRTEM, Energy dispersive X-ray spectroscopy, SAED and X-Ray diffraction studies. The antioxidant activity of the leaf extract of Gymnema sylvestre has been studied against a long lived 2,2-diphenylpicrylhydrazyl (DPPH) radical at room temperature.  The in-vitro MTT assay revealed a potent, selective, dose and time dependent anticancer activity of the freshly synthesized AuNPs on MCF-7 breast cancer cell lines and showed IC₅₀ at 30.47 μg/mL.