Development and Validation of HPLC Method for Determination of Triazophos Pesticide in Water

Abstract

While studying the fate of pesticide in different environmental segments such as soil and water, its accurate quantification is required. Therefore, development of suitable analytical method for determination of organophopsphorus pesticide residues in water is an important area of research. This study deals with the development and validation of HPLC method for determination of triazophos residues in water. The various HPLC parameters were optimized to obtain the clear and sharp chromatograms. The developed method was validated for accuracy, precision, linearity and robustness following the standard procedures. The results were found to be quite accurate and precise with standard deviation below 5%. The recovery varied from 92.1 to 108.5%. The limit of detection and limit of quantification were found to be 0.2449 and 0.8163 mg/L. The method was robust with respect to small change in mobile phase ratio and flow rate. But increase in flow rate decreased the retention time proportionately. The developed method can be used successfully without separation of pesticide from aqueous phase.